Journal: Scientific reports

Article Title: Microenvironment M1/M2 macrophages and tumoral progression vary within C57BL/6 mice from same substrain in prostate cancer model.

doi: 10.1038/s41598-024-65960-y

Figure Lengend Snippet: Figure 3. Quantification of M1 CD68 + iNOS + and M2 CD68 + CD206 + macrophages in murine prostate cancer tumor sections. (a) M1 DAPI + CD68 + iNOS + macrophage staining in prostate cancer tumor sections. (b) Comparison of total intratumoral M1 macrophages between mice from BL/6nA and BL/6nB nuclei *P = 0.0039. (c) Comparison of M1 macrophage populations between study regions for each group *P < 0.001. (d) M2 DAPI + CD68 + CD206 + macrophage staining in murine prostate cancer tumor sections (e) Comparison of total intratumoral M2 macrophages between mice from BL/6nA and BL/6nB nuclei. (f) Comparison of M2 macrophage subpopulations between study regions for each group *P < 0.001. Spleen. Macrophage staining control in spleen slice.

Article Snippet: For the M1 subpopulation, monoclonal rabbit anti-mouse iNOS antibody (NB300-605 Novusbio®) and donkey anti-rabbit IgG Alexa Fluor 647® antibody (Jackson Immunoresearch®) were used.

Techniques: Staining, Comparison, Control

Journal: Scientific reports

Article Title: Microenvironment M1/M2 macrophages and tumoral progression vary within C57BL/6 mice from same substrain in prostate cancer model.

doi: 10.1038/s41598-024-65960-y

Figure Lengend Snippet: Figure 4. M1, M2 and non-polarized macrophages population in the different tumor regions. (a) Representative image of the immunostaining for the detection of the M1, M2 phenotypes and non-polarized macrophages performed on the tumors obtained from the BL/6nA and BL/6nB groups. (b) Quantification of M1 phenotype macrophages (red), M2 (blue) and total macrophages (green) for each of the tumor regions studied in the BL/6nA group. (c) Quantification of M1 phenotype macrophages (red), M2 (blue) and total macrophages (green) for each of the tumor regions studied in the BL/6nB group. (d) Average percentage of M1 phenotype macrophages, CD68 + iNOS (red), M2 phenotype, CD68 + CD206 + (blue) and non-polarized macrophages CD68 + iNOS-CD206—(yellow) for each of the regions studied.

Article Snippet: For the M1 subpopulation, monoclonal rabbit anti-mouse iNOS antibody (NB300-605 Novusbio®) and donkey anti-rabbit IgG Alexa Fluor 647® antibody (Jackson Immunoresearch®) were used.

Techniques: Immunostaining

Journal: British Journal of Nutrition

Article Title: Treatment with oligonol, a low-molecular polyphenol derived from lychee fruit, attenuates diabetes-induced hepatic damage through regulation of oxidative stress and lipid metabolism

doi: 10.1017/s0007114511001322

Figure Lengend Snippet: Fig. 2. NF-kBp65 (A), cyclo-oxygenase-2 (COX-2) (B) and inducible NO synthase (iNOS) (C) expressions in the liver. m/m, Misty; db/db, diabetic; Veh, db/db vehicle-treated mice; Oligo-10, db/db mice treated with oligonol at 10 mg/kg body weight; Oligo-20, db/db mice treated with oligonol at 20 mg/kg body weight. Values are means (n 6 or n 10), with standard errors represented by vertical bars. a,b Mean values with unlike letters were significantly different (P,0·05; Duncan’s test).

Article Snippet: Rabbit polyclonal antibodies against PPARa, SREBP-1, SREBP-2, NF-kBp65 and RAGE, and mouse monoclonal antibody against cyclo-oxygenase-2 (COX-2) and inducible NO synthase (iNOS) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques:

Journal: British Journal of Nutrition

Article Title: Treatment with oligonol, a low-molecular polyphenol derived from lychee fruit, attenuates diabetes-induced hepatic damage through regulation of oxidative stress and lipid metabolism

doi: 10.1017/s0007114511001322

Figure Lengend Snippet: Fig. 5. Hepatic mRNA expressions of acetyl-CoA carboxylase (ACC) (A), fatty acid synthase (FAS) (B) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) (C). m/m, Misty; db/db, diabetic; Veh, db/db vehicle-treated mice; Oligo-10, db/db mice treated with oligonol at 10 mg/kg body weight; Oligo-20, db/db mice treated with oligonol at 20 mg/kg body weight. Values are means (n 6 or n 10), with standard errors represented by vertical bars. a,b Mean values with unlike letters were significantly different (P,0·05; Duncan’s test).

Article Snippet: Rabbit polyclonal antibodies against PPARa, SREBP-1, SREBP-2, NF-kBp65 and RAGE, and mouse monoclonal antibody against cyclo-oxygenase-2 (COX-2) and inducible NO synthase (iNOS) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: